The effect of Crocin against hypoxia damage of myocardial cell and its mechanism

Objective:To investigate the protective effect of Crocin against hypoxia damage of cardiac myocytes of neonatal rats and the regulation of HIF-1 and proly hydroxylase(PHDs). Methods: A model of CoCl2 simulated hypoxia damage was established in primary cultural myocardial cell. Expression levels of HIF-1α,VEGF,iNOS,as well as PHD1,2,3 protein in myocardial cells were detected by Western blot. Results: Compared with CoCl2 group,the viability of myocardial cell was significantly increased after treated 24 h at 10-5mol / L Crocin (P0.01),HIF-1α,VEGF and iNOS were expressed higher than those in Crocin+CoCl2 group(P0.01),the expression of PHD2 was significantly increased(P0.01),while the expression of PHD3 was remarkably reduced in Crocin+CoCl2 Group(P0.01). Conclusion: Crocin has better protective effect on hypoxic damage of myocardial cell. The mechanisms of protective effect of Crocin may be related to the activation of HIF-1-mediated pathway of the hypoxia response. PHDs may be involved in the pathophysiology regulated process of myocardial cells.