Construction of full-length cDNA library of yellow bud mutant leaves in Capsicum annuum L.using SMARTer technique

Total RNA was extracted from yellow bud mutant leaves of Capsicum annuum L.,and first-strand cDNA and ds cDNA were synthesized by LD-PCR technology.The purified ds cDNA was connected to vector pSMART2IFD,and the recombinant vectors were transformed into competent Escherichia coli cells DH5α by electroporation to construct full-length cDNA library of Capsicum annuum L..The library quality test results showed the titer of original library was 1.76×106 PFU/ml,the recombination rate was 94%,and the inserted fragment length was 500-2 000 bp,indicating that the library was ideal for target genes selection.