Solid state fermentation with recovery of Amyloglucosidase from extract by direct immobilization in cross linked enzyme aggregate for starch hydrolysis

Abstract Main limitations in application of Amyloglucosidase (AMG), an important industrial enzyme for starch hydrolysis, areits high production cost and limited reusability. Immobilization techniques have been tried to address the issue of reusability, however, these techniques either increase the cost of enzyme o rare difficult to scale up owing to special requirements for synthesis of carriers. We tried to address the issue of production cost and reusability for enzyme application by forming carrier free cross-linked enzyme aggregate (CLEA) of AMG from crude SSF extract of Aspergillus niger . CLEA recovered 65 percent activity from extract and retained 90% even after 25 cycles of reuse. CLEA showed improved affinity toward substrate; higher storage, thermal and pH stability. Use of cheap SSF and direct immobilization of AMG from extract can reduce the cost of starch hydrolysis.