Current developments in SELDI affinity technology

I. Introduction 34    II. Affinity Probes 35   A.  Initial Surface-Affinity Research 36   B.  Self-Assembled Monolayers 36   C.  Dextran 36   D.  Metal Affinity 36   E.  Antibody- and Protein-Affinity Probes 37   F.  Lectin Affinity Capture 38   G.  Inherent Hydrophobic Interaction Bio-Probes 38   H.  Bio-Molecular Interaction Probes 39  III. SELDI 39   A.  Fundamentals of SELDI 39   B.  SELDI in Proteomics 40  IV. Conclusions 41 References 41 The overall history and recent advancements in Surface-Enhanced Laser Desorption/Ionization (SELDI) affinity technology is reviewed. A detailed account of SELDI technology, utilizing Immobilized-Metal Affinity surfaces, pseudo-specific chromatographic surfaces, and biospecific interactive surfaces, is presented with particular emphasis placed upon examination of fundamental characteristics as well as specific applications for each. Finally, a detailed review of the specific use of such affinity surfaces in fundamental aspects of clinical, process, and research proteomics activity is presented. © 2003 Wiley Periodicals, Inc., Mass Spec Rev 23:34–44, 2004