Current developments in SELDI affinity technology
2004
I. Introduction 34
II. Affinity Probes 35
A. Initial Surface-Affinity Research 36
B. Self-Assembled Monolayers 36
C. Dextran 36
D. Metal Affinity 36
E. Antibody- and Protein-Affinity Probes 37
F. Lectin Affinity Capture 38
G. Inherent Hydrophobic Interaction Bio-Probes 38
H. Bio-Molecular Interaction Probes 39
III. SELDI 39
A. Fundamentals of SELDI 39
B. SELDI in Proteomics 40
IV. Conclusions 41
References 41
The overall history and recent advancements in Surface-Enhanced Laser Desorption/Ionization (SELDI) affinity technology is reviewed. A detailed account of SELDI technology, utilizing Immobilized-Metal Affinity surfaces, pseudo-specific chromatographic surfaces, and biospecific interactive surfaces, is presented with particular emphasis placed upon examination of fundamental characteristics as well as specific applications for each. Finally, a detailed review of the specific use of such affinity surfaces in fundamental aspects of clinical, process, and research proteomics activity is presented. © 2003 Wiley Periodicals, Inc., Mass Spec Rev 23:34–44, 2004
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
73
References
387
Citations
NaN
KQI