Expression and significance of bcl-2 gene in apoptotic muscle cells 103Pd radioactive stent bile duct in dogs

Objective To study the expression and significance of bcl-2 gene in the apoptotic muscle cells in γ-radiation-induced muscle cell lines.Methods bcl-2 gene in the control and r-radiation-induced apoptotic muscle cells was analyzed by using immunohistochemical technique.Results The bcl-2 gene transcript more highly expressed in general stent dog bile duct than in 103Pd radioactive stent dog bile duct,but apoptotic muscle cells were more highly in 103Pd radioactive stent dog bile duct than in general stent dog bile duct.Conclusion The low level of expression of bcl-2 gene may aid the effects tO understand the muscle cell sensitivity to γ-radiation apoptosis.103Pd radioactive stent may reduce the expression of bcl-2 gene in dog bile duct and prevent the biliary narrowing after dog bile duct injury by balloon. Key words: Apoptosis; 103Pd radioactive stent; γ-radiation bcl-2 gene