Targeted GSK3beta (glycogen synthesis kinase 3beta) gene RNA interference recombinant slow virus carriers and constructing, screening methods thereof

The invention relates to methods for constructing and screening targeted GSK3beta (glycogen synthesis kinase 3beta) gene RNA interference recombinant slow virus carriers and use thereof; RNA interference technology is used in the methods; two slow virus eukaryotic expression carriers capable of expressing in SH-SY5Y cells are constructed aiming at different target sequences of GSK3beta gene; a GP-Supersilencing-shGSK-3beta regrouping carrier, a pHelpher 1.0 carrier and a pHelper 2.0 carrier are used for cotransfection to 293T cells and cells are cultivated so as to obtain the carriers. According to the invention, the targeted GSK3beta gene RNA interference recombinant slow virus carriers can restrain GSK3beta gene expression in a high-efficient specificity manner and can be used for preparing GSK3beta relevant gene medicines capable of treating alzheimer disease.