Method of obtaining intermolecular conjugate for immunochromatographic determination of specific antibodies

FIELD: medicine. SUBSTANCE: invention relates to immunology and biotechnology. In immunochromatographic test-systems along with an analytic zone, intended for binding an analysable compound, usually, applied is a control zone, intended for binding the compound, conjugated with a label. The application of the control zone is necessary to control the functional activity of a reagent, conjugated with the label. If colouring of the control zone is absent, the result of the test is considered invalid. However, in case of the application of immunochromatography for determination of antibodies in blood with the label conjugated is a compound, intended for binding antibodies - further designated as CBAn (compound, binding antibodies). The concentration of antibodies in a sample often exceeds the concentration of CBAn, that is why the concentration of free molecules of CBAn is very low and the disappearance of the control zone in this case does not testify to the malfunction of the conjugate activity. To solve the said problem the claimed invention proposes to add antispecies antibodies against the antibodies sorbed in the control zone, to the conjugate In this case the control zone disappears only in case of loss of the CBAn activity. The antispecies antibodies are added in concentrations considerably lower than the binding ability of the conjugate, in order not to interfere with the interaction of the conjugate with the sample antibodies, as a result of which no considerable reduction of analysis sensitivity is observed. EFFECT: development of the method of obtaining intermolecular conjugates with colloidal gold, applied in immunochromatographic analysis. 3 dwg, 1 ex