Determination of ellagic acid content in cosmetics by reversed-phase high performance liquid chromatography

A reversed-phase high performance liquid chromatographic method for the determination of ellagic acid in cosmetics was established.The sample was analyzed on a Venusil XBP-C18 column(4.6 mm i.d×250 mm,5 μm) with UV 254 nm.The mobile phase consisted of V(CH3CN)∶V(H2O)∶V(CF3COOH)=20∶80∶0.08,at a flow rate of 1.0 mL/min.Results showed that as the concentration of ellagic acid is in the range of 3.98 μg·mL-1~79.6 μg·mL-1,the relationship between concentration and the corresponding peak area shows good linearity(R2=0.999 8).The regression equation can be expressed as A=203.83ρ-2.462 7;and the recoveries of ellagic acid achieve 96.5%~101.5%.The RSD of the method is 0.6%.