Expression changes of Survivin, BCRP and HER-2 genes after neoadjuvant chemotherapy of NE in breast cancer

Objective To investigate Survivin,BCRP and HER-2 genes prediction and sensitivity of breast cancer in neoadjuvant chemotherapy with NE ( Vinorelbine and Epirubincin).Methods There were 46 cases of breast cancer patients with neoadjuvant chemotherapy NE program,using reverse transcription-polymerase chain reaction (RT-PCR) method to detect Survivin,BCRP and HER-2 expression before and after chemotherapy and to analyze the differences in the relationship with chemotherapy.Results Neoadjuvant chemotherapy NE overall response rate was 76.1％ (35/46),which cCR accounted 15.2％ (7/46),pCR 10.9％ (5/46),cPR 60.9％ (28/46),SD 21.7％ (10/46),PD 2.2％ (1/46),respectively.After chemotherapy,positive expression rates of Survivin mRNA,BCRP mRNA and HER-2 mRNA decreased,the difference was statistically significant ( P ＜ 0.05).The efficient rate of the individual positive expression of Survivin mRNA,BCRP mRNA and HER-2 mRNA were lower than the negative groups ( P ＜ 0.05 ),three indicators of negative expression with chemotherapy were lower than other groups ( P ＜ 0.05).There was no relationship among the expression of Survivin mRNA,BCRP mRNA and HER-2 mRNA in breast cancer (P ＞ 0.05).Conclusion The combined of detection the expression of Survivin,BCRP and HER-2 in NE program is helpful to predict sensitivity of neoadjuvant chemotherapy in breast cancer. Key words: Breast cancer;  Neoadjuvant chemotherapy;  Survivin mRNA;  BCRP mRNA; HER-2 mRNA;  Prediction;  NE chemotherapy