COMPOSITIONS AND METHODS FOR MAKING AND DECODING PAIRED-GUIDE RNA LIBRARIES AND USES THEREOF

The present disclosure relates to compositions and methods for making and decoding paired-guide RNA (pgRNA) libraries using the Clustered Regularly-Interspaced Short Palindromic Repeats (CRISPR) system, and using the resulting pgRNA/CRISPR libraries to identify genetic interactions or functional non-coding elements.