РЕНТГЕНО-МОРФОЛОГИЧЕСКИЕ ПАРАЛЛЕЛИ ОСТЕОРЕГЕНЕРАТИВНОГО ПРОЦЕССА ПРИ ИСПОЛЬЗОВАНИИ ПРЕПАРАТА НА ОСНОВЕ ЭТИНДРОНАТОВ ИОНОВ ЛАНТАНОИДОВ

Objective – to study the effect of the agent based on ion lanthanide etidronate and calcium on the process of osteoregeneration in the experimental model of the tibial defect. Methods. The experiment included 36 rabbits with the experimental tibial damages and subsequent introduction (on the days 3 and 5) of the agent based on ion lanthanide etidronate and calcium with the dosage of 0.2 ml in the main group (n = 18) and without it in the comparison group (n = 18). The roentgen metric and morphological analyzes of the bone tissues, which filled the perforative hole, were performed on weeks 1, 4 and 8 of the experiment. A morphometric method was used in order to quantify the areas of the studied structures. A qualitative assessment of osteoblastic activity was carried out using the cell line MC3T3-E1, Subclone 4 (ATCC® CRL-2593 ™) in order to determine the mechanism of action of the drug. Results . The studied agent stimulated the healing process of the bone defect already on the day 7 of the experiment that consisted in a decrease of the area of the perforative hole and the volume of leukocyte-necrotic masses. On the fourth week, it caused the closure of the defect by the membrane reticulated bone. At the end of the experiment, in the area of experimentally induced of trauma, the formed lamellar bone could be visualized. The authors found that the tested compound induced the osteoblastic cell activity. Conclusion . The use of the drug based on lanthanide etidronate and calcium is effective in the early stages of healing of small bone defects.