A portable droplet generation system for ultra-wide dynamic range digital PCR based on a vibrating sharp-tip capillary.

Abstract Monodisperse droplet has been widely used as a versatile tool in different disciplines including biosensing. Existing methods still struggle to balance the droplet generation performance with system simplicity. Here we introduce a novel droplet generation scheme based on the acoustic streaming generated from a vibrating sharp-tip capillary. The unique fluid pattern enables efficient droplet generation without any external pressure sources. This method achieved real-time modulation of droplet size over an ultra-wide range (6.77–661 μm), high throughput (up to 5000 droplets/s), and good monodispersity (