Analysis of the Large Intestine and Cecum Microflora in Wild House Mouse (Mus musculus castaneus)

Current microflora cultivating methods can only retain a very small percentage of microflora species, which has constrained the research of microorganisms. Researchers have developed many different methods to overcome the difficulties. T-RFLP, the method used in this study, is considered one of the most efficient. This study employs both T-RFLP and direct DNA sequencing to analyze the 16s rDNA of microfloras in the large intestine and cecum of a wild house mouse to reveal the microflora composition of the gastrointestines. I found significant differences between the compositions of microfloras in the large intestine and cecum.