An investigation of antitumor activity of mice serum treated with selenium-protein polysaccharide

Objective:To explore the effect and mechanism of serum treated with selenium-protein polysaccharide(SPP),extracted from rich-selenium Agaricus blazei,inducing apoptosis of K562 cells in vitro and compare the difference of antitumor activity of serum treated with SPP between normal and pathological mice.Methods:According to serum pharmacology,serum treated with SPP was prepared.Growth inhibition rate of K562 cells was detected by MTT method.Apoptotic phenomenon was observed under a fluorescent microscope and through DNA electrophoresis,respectively.Caspase-3 enzymatic activity was measured by colorimetry.Results:Serum treated with SPP(10%)could significantly inhibit the growth of K562 cells.There was a markedly positive correlation between drug dose(50,100 mg·kg-1),time(24～48 h)and inhibitory rate.Apoptotic phenomenon was showed by morphology and DNA electrophoresis.Compared with control group,Caspase-3 gene was significantly up-regulated.Compared with the serum treated with SPP of nonbearing tumor group,that of bearing tumor group has much more potent effect on antitumor.Conclusion:Serum treated with SPP could induce apoptosis of K562 cells.Its mechanism may be related to up-regulation of Caspase-3.