[Experiments to replace asparagine in Sauton's medium with organic acids in producing PPD tuberculins].

Comparative investigations were carried out on the changes in the blood serum of normal and diseased rainbow trout with virus hemorrhagic septicaemia. Determined was the amount of the total protein and the protein fractions. The electrophoretic investigation of blood serum of rainbow trout revealed one dominating albumin fraction, 3 alfa-globulin fractions, 2 beta-globulin ones, and one gamma-globulin one. The following up of the protein fraction dynamic during the various seasons did not reveal any dependence on the temperature curve. Rainbow trout manifesting clinically virus hemorrhagic septicaemia showed lowering of the total protein amount and changes in the electrophoretic profile and the quantitative ratio of the protein fractions. There were a drop of the albumin percent and a rise of the beta- and gamma-globulin fractions. This led to a change in the coefficients alfa:beta (0.89) and albumin: globulin (0.14).