The Formation of an R-prime Carrying the Fraction I Dehalogenase Gene from Pseudomonas putida PP3 using the IncP Plasmid R64.44

SUMMARY: Plasmid R68.44 was transferred to Pseudomonas putida PP3 at a frequency of approximately 10-6. In the new strain, P. putida PPW1, the plasmid, designated pUU1, lost a 2.0 kb fragment corresponding to IS21 which had previously been implicated in enhanced chromosome mobilization, but gained between 3.4 and 5.7 kb of DNA. Plasmid pUU1 was used to mobilize the fraction I dehalogenase gene at a low frequency of approximately 10-1 into another strain of P. putida. The R-prime plasmid, designated pUU2, contained an additional 9.2 to 11.0kb fragment which carried the fraction I dehalogenase.