Murine neutrophils collected from subcutaneously implanted sponges

Abstract Polyvinyl sponges implanted subcutaneously in mice were used as a source of murine neutrophils (PMNs). Yield of PMNs from sponges averaged 6.3 × 10 5 PMNs per mouse and viability of sponge PMNs was 96.5%. These results were comparable to the yield and viability of PMNs harvested from peripheral blood. The chemotactic activity of sponge PMNs was significantly greater than the chemotactic activity of peripheral blood PMNs (chemotactic index of 4.78 ± 1.66 in sponge PMNs versus 2.16 ± 1.08 in peripheral blood PMNs, P