Changes of serum proteins in patients with cervical cancer after radical hysterectomy and pelvic lymphadenectomy

OBJECTIVE:To compare the changes of significant serum proteins before and after the operations of radical hysterectomy and pelvic lymphadenectomy for patients with cervical cancer and seek for a group of serum biomarkers that can be used to evaluate the curative effect of cervical cancer. METHODS: Surface enhanced lazar desorption/ionisation-time of flight-mass spectrometry ( SELDI-TOF-MS ) with immobilized metal affinity capture arrays-Cu (IMAC-Cu) was used to test the serum of 49 patients with cervical cancer and 71 age-matched healthy women. A group of significant proteins in classification were selected from the data of proteomic spectra by a Biomarker pattern software and Biomarker Wizard software. The serum samples from 35 cervical cancer patients, at the tenth day and 3 month after the radical hysterectomy and pelvic lymhpadenectomy, were tested as well. This group of proteins were compared as biomarkers. RESULTS: Forty-seven proteins were detected with a significant level of P0.05, from group A. Six proteins with M/Z value of M8929.31,M7930.52,M9127.31,M8141.01,M7963.06 and M9280.63 had high scores in building a model of decision tree classification algorithm for invasive cervical cancer detection. The sensitivity and specificity of M/Z value of M8929.31 were 97.96% and 98.59% respectively. The 6 proteins, which appeared to be down regulated in patients with invasive cervical cancer, had gradually retrieved in a level of P0.05 after the surgery, except M/Z value of M9280.63, and continuously climbed in a level of P0.05 at the time of 3 month postoperation including M9280.63. CONCLUSIONS: The group of biomarkers with M/Z value of M8929.31,M7930.52,M9127.31,M8141.01, M7963.06 and M9280.63 might potentially be of value regarding the monitoring of therapy response, and predicting the prognosis of cervical cancer.