Quality Control of DNA Template in RAPD Technique

In this report,the RAPD technique has been applied to analyze the niddle boipsy specimens from different tumor patients.We noted that the measurement errors of RAPD was buffered differentiy with various DNA extraction methods due to limited templates,thus resulted in the distinct profiles of RAPD.We failed to amplify templates obtained by hot base (KOH) treatment.The multiple bands were demonstrated for target DNAs,when proteinase K/phenol extraction or single vial extracting procedure were applied.Superior to phenol extraction,however,using single vial extraction method to prepare template could improve the sensitivity and polymorphisms of RAPD.