Killing of Klebsiella pneumoniae mediated by conjugation with bacteria carrying antibiotic-resistance plasmids of the group N.
1981
Abstract Escherichia coli K-12 strains carrying any one of a number of different plasmids of the incompatibility group N have been found to kill Klebsiella pneumoniae , M5a1. A simple spot test is described that could be of value in diagnosing the presence of these plasmids. Killing is closely associated with the ability of the N + strains to mate with M5a1 and all conjugation-deficient (Tra − ) mutants were also unable to kill M5a1 (Kil −1 ). At a low frequency, some Kil − mutants could be isolated that showed little or no transfer deficiency. Plasmids of the groups P and W which have been suspected (on other grounds) to specify conjugative systems interrelated in some manner to that specified by N plasmids, also kill M5a1 but to a lesser degree.
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