Microarray-based genomic selection for high-throughput resequencing

We developed a general method, microarray-based genomicselection (MGS), capable of selecting and enriching targetedsequences from complex eukaryotic genomes without the repeatblocking steps necessary for bacterial artificial chromosome(BAC)-based genomic selection. We demonstrate that largehuman genomic regions, on the order of hundreds of kilobases,can be enriched and resequenced with resequencing arrays.MGS, when combined with a next-generation resequencingtechnology, can enable large-scale resequencing in single-investigator laboratories.Technological innovation in DNA sequencing offers the promise ofa more comprehensive, cost-effective and systematic ascertainmentof genetic variation