Anti-Interleukin-10 Unleashes Transcriptional Response to Leishmanial Antigens in Visceral Leishmaniasis Patients.

Visceral leishmaniasis (VL; Leishmania donovani) cases produce interferon-γ and tumour necrosis factor in response to soluble leishmanial antigen (SLA) in whole blood assays. Using transcriptional profiling, we demonstrate the impact of interleukin-10 (IL10), a cytokine implicated in VL, on this response. SLA stimulation identified 28 differentially expressed genes (DEGs), 17/28 in a single network with TNF as hub. SLA plus anti-IL10 produced 454 DEGs, 292 in a single network with TNF, IFNG, NFKBIA, IL6 and IL1B as hubs in concert with a remarkable chemokine/cytokine storm. Our data demonstrate the singular effect of IL10 as a potent immune modulator in VL.