DIRECT SOMATIC EMBRYOGENESIS OF A BIODIESAL PLANT JATROPHA CURCAS (LINN.)

Present research work was initiated on induction of direct somatic embryo genic callus from cotyledon explants in important biodiesel and medicinal plant Jatropha curcas. These explant inoculated on MS medium supplemented with various concentration of growth hormone like, BAP, IAA, and GA3 addition with proline and PEG.MS media containing 2, 4- D in combination of BAP were able to initiation of callus. MS medium containing BAP in various concentrations viz 0.2, 0.4, 0.6, 0.8 mg/l with combination different concentrations of GA3like 0.2, 0.4, 0.6, 0.8 and1.0 mg/l,IAA 0.1, 0.2, 0.3 mg/l and proline observations were also recorded. Induced callus from cotyledon explants were using Murashige and Skoog (MS) medium supplemented with 0.5 mg/l BAP, 0.6 mg/1 gibberellic acid (GA3), and proline was able to produce direct somatic embryo genic callus. This method regeneration of plant was more effective as compare to other methods because regeneration percentage 70% -80% respectively. The percentage of embryo maturation was observed after three weeks and the matured somatic embryos were subculture on hormone free MS medium for plant regeneration.