Proteomic Analysis of Stripe Rust Resistance Wheat Line Taichung29*6/Yr5 Inoculated with Stripe Rust Race CY32

【Objective】 To investigate the biochemical changes of wheat resistance to stripe rust after infected with the stripe rust,a resistive line of wheat(Taichung29*6/Yr5) infected with stripe rust CY32 was compared to mock-inoculated wheat by comparative proteomics technology.【Method】 Proteins were extracted from leaves infected with a virulent race of stripe rust CY32 and control group after 48 hours of inoculation,and then separated by using two-dimensional electrophoresis(2-DE).The differential protein spots were selected for MALDI-TOF MS analysis and database searching.【Result】 By using 2-DE,2 uniquely expressed spots,5 up-regulated spots and 6 down-regulated spots were observed in Taichung29*6/Yr5 leaves inoculated with stripe rust CY32.Total of 11 proteins,including pathogenesis-related homeodomain protein,beta-glucosidase,glutathione transferase etc,were identified.【Conclusion】 Through analysis of protein function,it is presumed that these differential proteins are correlated with the infextation of stripe rust race CY32.