Total Internal Reflection Fluorescence Sensing - Quality Assurance and Application to Water Analysis

Based on the water directives of the European Union, the results of a biosensor system using total internal reflection fluorescence (TIRF) are demonstrated. The optical principal, the assay type, as well as the calibration and validation parameters are discussed. Results for endocrine disrupting compounds are given, and the validation parameters are outlined. These are applied to a measurement in comparison to a collaborative test in cooperation with accredited water laboratories using high performace liquid chromatography (HPLC) and gas chromatography (GC) techniques.