Biomolekulare Interaktionen: Manche mögen’s heiß

The Microscale Thermophoresis (MST) is a biophysical method to characterize biomolecular interactions in bulk solution. Interaction affinities can be quantified in biological liquids, without immobilization and without a size or mass limitation. Its material consumption is very low and today’s resolvable dissoziation constants range from Kd = 100 pM to Kd = 10 mM.