A method for extracting mitochondrial polysomes and Its Application

The present invention discloses a method and its application in mitochondrial extracts of polysomes. The method of extraction of polysomes mitochondria comprising the steps of: extracting mitochondrial target cell extraction buffer with mitochondria; ribosome extraction buffer and then lysed mitochondrial ribosomal obtained suspension; mitochondrial ribosomal suspension was divided into two equal portions, one parts with an RNA enzyme treatment, no treatment as a further control; 10-30% sucrose density gradient formulated, will balance mitochondrial ribosomal upper suspension plated sucrose density gradient, 4 ℃? Centrifuged at 180000 × g for 2-4 hours; from top to bottom components were collected by centrifugation, RNA and protein extracts were analyzed to determine the components where the component polysomes. The present invention may be apparent detected polysomes mitochondria and mitochondrial mRNA polysomes component was quantitatively analyzed to obtain translational level mitochondrial genes to be studied.