肾癌G250抗原蛋白多糖(PG)区蛋白cDNA的克隆和序列测定

Objective To obtain the cDNA of proteoglycan (PG) region protein of G250 antigen to get ready for preparing the hybridoma and monoclonal antibody of G250 antigen. Methods The total RNA was extracted from human renal carcinoma cell line 786-0 and the intact cDNA of PG region protein was amplified by RT-PCR. The cDNA was cloned into pCA13 vector and transformed into E. coli JM109. Results The sequence determined was completely consistent with the published PG region protein cDNA. Conclusion PG region protein cDNA has been cloned successfully.