Cell membrane chromatography coupled online with LC-MS method to screen anti-anaphylactoid components from Magnolia biondii Pamp. targeting on Mas-related G protein-coupled receptor X2.

Mas-related G protein-coupled receptor X2 was a mast cell specific receptor mediating anaphylactoid reactions by activating mast cells degranulation, it was also identified as a target for modulating mast cell-mediated anaphylactoid and inflammatory diseases. The anti-anaphylactoid drugs used clinically disturb the partial effect of partial mediators released by mast cells. The small molecule of Mas-related G protein-coupled receptor X2 specific antagonists may provide therapeutic action for the anaphylactoid and inflammatory diseases in the early stage. In this study, the Mas-related G protein-coupled receptor X2 high expression cell membrane chromatography was coupled online with LC-MS and successfully used to screen anti-anaphylactoid components from Magnolia biondii Pamp. Fargesin and Pinoresinol Dimethyl Ether were identified as potential anti-anaphylactoid components. Bioactivity of these two components were investigated by beta hexosaminidase and histamine release assays on mast cells, and it was found that these two components could inhibit beta hexosaminidase and histamine release in a concentration dependent manner. This Mas-related G protein-coupled receptor X2 high expression cell membrane chromatography coupled online with LC-MS system could be applied for screening potential anti-anaphylactoid components from natural medicinal herbs. This study also provided a powerful system for drug discovery in natural medicinal herbs. This article is protected by copyright. All rights reserved.