火薬成分の毒性に関する研究 (その3) : 皮膚吸収に関する実験的研究

In order to see the effect of nitroglycerine and nitroglycol absorbed through the skin, each auricle of rabbits was smeared by 3 grams of the substance, i.e. 6 grams were used for each rabbit. And, the smeared surface was covered tight by cellophane tape and left for 1-2 months, during which appearance of Heinz bodies in the peripheral circulation was examined. After this period the animals were killed and post mortem histological changes were examined. 1. The same changes as in the case of subcutaneous injection, i.e., vacuolization and formation of hyaline transverse bands of the cardiac muscle were noticed. 2. Local skin smeared by the substance showed hyperkeratinization, acantosis and nail-leg elongation and roundcell infiltration, mainly of plasma cells. 3. Hypoplasia of the bone marrow, roundcell infiltration in the Glisson's sheath of the liver, hyperemia of the spleen, etc were noticed. 3. Two weeks after the smearing, red cells with Heinz bodies were observed in the circulating blood.