Ultrasonic Extraction of Lycorine and Galanthamine from Red Spider Lily Bulb (Lycoris radiate)

Optimal conditions for the ultrasonic extraction of lycorine and galanthamine from red spider lily bulb were established in this study.The compounds were determined simultaneously by HPLC.The effects of extraction time,temperature,ultrasonic treatment time and material-to-solvent ratio on extraction efficiency were investigated.Analysis of variance showed that three extraction parameters in the decreasing order of importance were extraction time,ultrasonic radiation time and temperature.An extraction temperature of 70 ℃ and 2.5 h extraction followed by 30 min ultrasonic treatment repeated in sequence twice proved optimal,which provided maximum yields of lycorine and galanthamine,0.1561% and 0.0253%,respectively.