Cloning and characterization of an interstitial deletion at chromosome 11p15 in a sporadic breast cancer

In a number of types of cancer including breast, hepatocellular, and bladder carcinoma, frequent losses of heterozygosity (LOH) on chromosome 11p15 have indicated the presence of one or more tumor suppressor genes in this region. In the present study, we report the detection and characterization of a rearrangement at 11p15 in a sporadic breast carcinoma. Genomic DNA clones encompassing the rearranged region were isolated; localization of both flanking clones to 11p15 by two-color fluorescent in study hybridization (FISH) indicated that the rearrangement was caused by an interstitial deletion in the affected allele