Identificación de nematodos fitoparásitos asociados al cultivo de rosa (rosa sp), en el sector lasso provincia de cotopaxi
2018
he present research work was carried out with the objective of identifying the different species of phytoparasitic nematodes that are associated with the cultivation of rose (Rosa sp), in the Lasso sector, Cotopaxi province, Saquimalag neighborhood through the respective soil sampling in four varieties of rose cultivars.
For the investigation, samples were collected and taken to the laboratory to be analyzed by extraction. The well-known method of the Baerman funnel and the Centrifuging method, root damage was analyzed as physiopathies by extracting the plant in its entirety, the pure complete root sample was collected. The morphometry of the specimens was prepared semi-permanent microscopic sheets and were observed under a phase contrast microscope (leica DM1000) and finally the identification of the species, the adult state of the nematode was taken into account to analyze body length, stylet, head and tail, gender identification according to the type of esophagus, tail size of the nematode and type of stiletto.
In the research, no experimental design was applied since the culture was established, the laboratory study was carried out, taking into account the factors under study of four varieties of rose cultivars and as treatments two flower farms, for the data analysis was used statistical programs and related to mathematical and percentage calculations
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