Morphological changes during the formation of amoebic liver abscess in vagotomized hamsters
2020
Amoebic liver abscess (ALA) is the main
extra-intestinal complication caused by Entamoeba
histolytica. Given the histological features of ALA in
hamsters and the importance of the vagus nerve in the
immune response, the aim of this study was to identify
and analyze the major changes in ALA that are caused
by a vagotomy. The changes found are related to
inflammatory foci and abscess size, the type of collagen
formed, and the number of trophozoites in lesions. Male
hamsters were divided into three groups: Intact animals
(IA) and those undergoing a false operation (SHAM) or
a subdiaphragmatic vagotomy (VAG). In each group, E.
histolytica trophozoites or culture medium (CM) were
inoculated in hamsters by the intrahepatic route, and
then euthanized at 6h, 12h, 24h, 48h, 4d or 7d postinfection. Initially the growth of the abscess was more
rapid in the VAG group, but at day 7 it was faster in the
IA and SHAM groups. VAG animals showed a higher
quantity of type III collagen than the IA and SHAM
groups. A larger number of amoebic trophozoites/mm2
was observed up to day 4 in VAG hamsters (23.3±2.19)
compared to IA (14.6±0.23) and SHAM (6.13±0.87)
animals. This parameter decreased by day 7 in VAG
(13.4±0.87) with respect to IA (24.7±1.47) and SHAM
(21.7±1.48). The results show that a subdiaphragmatic
vagotomy influenced the development of ALA in
hamsters, suggesting a modification of the
morphological structure of damaged hepatic tissue.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI