Identification and Separation of the Novel Antifungal Antibiotic of Streptomyces Hygroscopicus BOS-013 Strain by Simulated Moving Bed Chromatography

BOS-013 actinomycete strain was obtained by separating difference soil of areas from ChangBai Mountain. The antifungal antibiotic produced by Streptomyces Hygroscopicus BOS-013 was firstly purified by means of macro-porous adsorbent resin and thin-layer chromatography. Separation and Purification of the antifungal antibiotic from its fermentation broth of streptomyces hygroscopicus BOS-013 strain were further carried out by Simulated Moving Bed Chromatography and then the crystal of the antibiotic with high purity was got. In this paper, the methods of purification by adsorbing of micro-porous adsorbent resin and detection by high performance liquid chromatography were established. The exact structure of the antibiotic was identified by mass spectrometry and NMR spectra.