Antioxidative Activities and Inhibition of DNA Damage of Ethylacetate Fraction from Sophorae Flos and Sophorae fructus

ABSTRACT Objectives : In this study, we demonstrated the antioxidant activities and the inhibitory effect on oxidative DNA damage of ethyl acetate fractions extracted from Sophorae Flos and Sophorae fructus.Methods : Sophorae Flos and Sophorae fructus were extracted with methanol(MeOH) and divided to Petroleum ether, Ethyl acetate(EtOAC) and Water fraction. The antioxidant activities were conducted by the 1,1-diphenyl-2-picryl hydrazyl(DPPH) radical, 2, 2'-Azine-bis(3 -ethylbenzothiazoline-6 sulfonic acid) diammonium salt(ABTS) radical scavenging assay, Fe 2+ chelating assay and Reducing power assay. The inhibitory effect of DNA damage were characterized on φX-174 RF I plasmid DNA cleavage assay. In addition, we analyzed the Total phenol contents and the Vitamin C content s of Sophorae Flos and Sophorae fructus.Results : The results of DPPH were 92.71% and 94.72%, ABTS were 87.16% and 62.44%, and Fe 2+ chelating were 95.81% and 85.11% at 200㎍/㎖ of Sophorae Flos and Sophorae fructus respectively. The Sophorae Flos showed stronger effect than Sophorae fructus in Reducing Power assay. Total phenol content was 111.77 ㎎/g and 122.54 ㎎/g, and Vitamin C content was 2.59 ㎎/g and 3.03 ㎎/g . Also both Sophorae Flos and Sophorae fructus have inhibitory antioxidant effect on φX-174 RF I plasmid DNA cleavage assay.Conclusions : Over all, this study suggests that Sophorae Flos and Sophorae fructus can be used as not only effective antioxidant but also natural medicine.Key words : Sophorae Flos, Sophorae fructus, DPPH, Reducing power, DNA damage, Fe