Cloning, Characterization, and Chromosome Mapping of RPS6KC1, a Novel Putative Member of the Ribosome Protein S6 Kinase Family, to Chromosome 12q12–q13.1 ☆

Abstract A novel cDNA encoding a putative Ser/Thr protein kinase was isolated from a human skeletal muscle cDNA library. It contains an open reading frame that extends from nt 104 to 1510 and codes for a protein of 469 amino acids. A catalytic domain containing the conserved residues of the Ser/Thr protein kinase, especially human ribosome protein S6 kinase (RSK), was found to be located in the C-terminal end of the deduced protein. The gene was mapped to human chromosome 12q12–q13.1 by fluorescence in situ hybridization, and this result was confirmed with the Radiation Hybrid GB4 panel. Northern hybridization showed that the novel gene is expressed in all 16 human tissues tested with especially strong expression in testis, skeletal muscle, and brain, whereas weak expression was detected in kidney, thymus, small intestine, liver, lung, heart, and colon.