Exclusion of paternity by DNA analysis in addition to blood group testing in a family case.

: Three men, all related to the mother were involved in a paternity case. Only one of them, an uncle of the mother, could be excluded by conventional blood testing of 22 polymorphic systems including HLA and Gm. Because it was not possible to exclude the other men (the brother and the father of the mother) by these systems restriction fragment length polymorphisms as detected by the probes YNH24 and hMF1 were analyzed. The results with probe hMF1 did not reveal additional information. Both Taq I and Pst I digests, probed with YNH24 excluded the father of the mother from paternity of the child under investigation.