Coupling of transcription and translation as the factor regulating the transcription of genes rpoBC in Escherichia coli cells

: The natural transcription polarity of proximal and distal elements of the rplJL-rpoBC operon is increased when translation is inhibited in Escherichia coli cells. It is shown that transcription uncoupling to translation terminates within EcoRI-2,6 fragment of the operon which contains the transcription attenuator. We suggest that transcription attenuation in the rplJL-rpoBC operon is regulated by the coupling of transcription to translation of the intergenic fragment which overlaps the attenuator sequence.