[Influence of high mobility group box 1 on migration of human cord blood CD34(+) cells].

The objective of study was to explore the influence of high mobility group box 1(HMGB1)on migration of cord blood CD34+ cells and their mechanism of migration.The expressions of receptor for advanced glycation end products(RAGE),toll-like receptor-2(TLR2)and TLR4 were detected by flow cytometry.The CD34+ cells in umbilical cord blood(CB)were enriched by MiniMACS and were exposed to various concentration of HMGB1(10,50,100,1,000 ng/ml),then the migration effect of HMGB1 on umbilical cord blood(UCB)CD34+ cell count was determined by microscopy,the chemotactic index was calculated.The CD34+ cells untreated with HMGB1 were used as control.The results indicated that the purity of the isolated CD34+ cells was more than 98%.The HMGB1 could promote the migration of CD34+ cells,and the migration effect of HMGB1 on CD34+ cells in certain concentrations gradually increased along with raise of concentration,the strongest effect was observed in concentration of 100 ng/ml,there was significant difference as compared with control(p0.01).Anti-RAGE antibody partially inhibited the migration effect of HMGB1 on CD34+ cells.It is concluded that the HMGB1 in certain concentration can enhance migration of CD34+ cells,which may be mediated through RAGE.