Association of GnRH receptor gene polymorphism on testicular parameters and semen quality in HF crossbred bulls

The present research was conducted to study the association of GnRH receptor gene polymorphism on testicular parameters and semen quality in fortyfour HF crossbred bulls. Two different genotypes GG and GA having amplification size 170, 118, 96 and 214, 170, 118 were identified, respectively. Genotypic frequencies were 0.82 (GG) and 0.18(GA) and gene frequency of G allele was 0.91 and for A allele was 0.09. The testicular parameters like scrotal circumference (SC), testicular width (TW), testicular thickness (TT) were non significant while testicular length (TL) was significant (P<0.05) among two genotypes. Seminal attributes like semen volume (Vol) concentration (Conc.) initial motility (IM), post thaw motility (PTM), abnormal sperm rate (ASR) and acrosome integrity test (AIT) were non significant while hypo osmotic swelling test (HOST) was significant (P<0.05) among two genotypes.