[The effects of shRNA-CXCR4 on breast cancer cells migration and invasion].

Objective To study the effect of shRNA-CXCR4 on human breast cancer cells migration and invasion.MethodsThrough knockdown CXCR4 by shRNA,the CXCR4 mRNA expression and protein expression were examined with RT-PCR and Western blot separately.The matrigel penetrating ability of human breast cancer cells was examined in vitro experiments.ResultsThe CXCR4 mRNA express and its protein express decreased significantly in MCF-7,MDA-MB-231 and MDA-MB-435s breast cancer cells(P0.05).The invasive ability of human breast cancer cells was significantly decreased(P0.05).Conclusionthrough knockdown CXCR4 by shRNA,the invasive ability of human breast cancer cells can be inhibited obviously.