Effects of Inhaled PAF in Humans on Circulating and Bronchoalveolar Lavage Fluid Neutrophils: Relationship to Bronchoconstriction and Changes in Airway Responsiveness

We have compared the effect of inhaled platelet activating factor (PAF) on circulating neutrophils with its ability to induce bronchoconstriction and bronchial hyperresponsiveness in humans. Human volunteers inhaled PAF, given as six successive inhalations 15 min apart, followed by bronchoalveolar lavage (BAL) 4 h later. The mean density and volume of circulating neutrophils were measured by metrizamide gradients and flow cytometry, respectively. PAF caused a decrease in Vp30 of 38.2 ± 4.5% at 5 min after the first inhalation (p < 0.001). This was associated with a fall in the peripheral blood neutrophil count from 3.15 ± 0.3 to 1.1 ± 0.3 × 106 per ml (p < 0.001), followed by a rebound neutrophilia (p < 0.01). The mean density of peripheral blood neutrophils fell significantly at 15 min (p < 0.02), with a return to baseline values despite further PAF inhalations; this was associated with an increase in neutrophil volume (n = 4; p < 0.05). The numbers of neutrophils (× 105) in BAL fluid after PAF were sign...