The Effect of a Two-Hour, Room Temperature Incubation of Human Spermatozoa in TEST-Yolk Buffer on the Rate of Fertilization in Vitro

Patients: To reassess the use of TEST-yolk buffer (TYB) in an in vitro fertilization (IVF) program by comparing fertilization rates achieved in a glucose-free cleavage medium by the standard IVF preparation of sperm versus a 2-h, room temperature incubation of sperm in TYB.