Az ACC szintáz-(1-aminocyclopropane-1-carboxylate synthase) gén expressziójának vizsgálata a szamóca hazai fajtáiban és azok antiszensz ACC-szintáz génnel transzformált vonalaiban = Examination of ACC-synthase (1-aminocyclopropane-1-carboxylate synthase) gene expression in strawberry cultivars produced in Hungary and in their lines transformed with antisense ACC-synthase gene

A cDNS-AFLP technika alkalmazasaval a szamoca eresere jellemző uj es utoerő gyumolcsokben mar leirt, eres indukalta geneket azonositottunk. Sikerult elkuloniteni eresi stadiumokra, valamint szovettipusra jellemző szamoca geneket. Az izolalt klonokban vannak olyan potencialis szabalyozo elemek, amelyek klimakterikus es nem klimakterikus gyumolcsokre egyarant jellemzőek. Ezek funkcionalis elemzese fenyt derithet a nem klimakterikus eres szabalyozasanak ismeretlen lancszemeire. Az etilen bioszintezis es jelatviteli utvonal kulcsgenjei a szamocaban is jelen vannak. Elsőkent szamolunk be szamoca ACC-szintaz (FaACS) es CTR1 gen izolalasarol. Az altalunk azonositott FaACS szerepe valoszinűleg hasonlo az almabol izolalt MdACS5-ehez, ugyanis fiatal szovetekben es a zold gyumolcsben irodik at, elicitorokkal is indukalhato. Az ACS-el ellentetben a FaACO eres indukalta mintazata arra enged kovetkeztetni, hogy az eres soran az etilentermeles a szamocaban is nő. Az etilen valasz jelatviteli utban negativ regulatorkent műkodő CTR1-et kodolo FaCTR1 expresszioja a mas novenyekből izolalt CTR1-ekhez hasonloan konstitutiv. Az izolalt ACS, ACO es CTR1 genek es promotereinek bioinformatikai elemzese sok uj informacioval jarul hozza a szamoca etilen bioszintezis es jelatviteli folyamatok tisztazasahoz, de az etilen szamocaban betoltott szerepenek pontos meghatarozasahoz szukseges az ACC-szintaz es -oxidaz gencsalad tovabbi tagjainak izolalasa, expresszios mintazatuk es promotereik meghatarozasa. | New ripening specific strawberry genes were isolated by cDNA-AFLP technique together with genes inducible by fruit maturation and already described in climacteric fruits. Strawberry genes related to ripening stages and tissue types could be discriminated. Potential regulatory elements characteristic both for climacteric and non-climacteric fruits were identified in the clones. Their functional analysis can help to clarify the unknown molecular elements of non-climacteric ripening. Key genes of ethylene biosynthesis and signal transduction are present in strawberry. We are the first, who report about isolation of ACC-synthase (FaACS) and CTR1 genes from strawberry. Role of FaACS obtained in our experiments, is probably similar to the apple derived MdACS5, its transcript can be found in young tissues and green fruits, and can be induced by elicitors. Ripening-related induction of FaACO suggests that ethylene production increases in strawberry during maturation process. Expression of CTR1 functioning as negative regulator in ethylene signal transduction is constitutive like in other plant species. Bioinformatic analysis promoters of isolated FaACS, FaACO and FaCTR1 provides valuable new information for learning ethylene biosynthesis and signal transduction in strawberry, but for determination of actual role of ethylene in strawberry ripening it is necessary to isolate further members of ACS and ACO multigene families, to characterize their promoters and expression patterns.