Diversidad de especies de Banana streak virus en híbridosinterespecíficos de Musa spp. cultivados en Cuba

In 2009, Banana streak virus (BSV) species: Obino L’Ewai (BSOLV), Goldfinger (BSGFV) and Imove (BSIMV) were detected in Cuba, on several samples collected from FHIA 18, FHIA 21 and FHIA 25 cultivars. It is known that AAB and AAAB genotypes are prone to infect with BSV due to activation of endogenous infectious BSV (eBSVs) integrated in the nuclear genome. Hence, it was necessary to explore the diversityand distribution of BSV species on interspecific hybrids grown in Cuba. To this aim, surveys were undertaken in production areas of ten provinces. Leaves samples from symptomatic or asymptomatic plants of 13 cultivars of AAB, AAAB, AABB and ABB genotypes, were collected randomly and indexed by multiplex-immunocapture-PCR with primers of species BSOLV, BSGFV, Mysore (BSMYV) and BSIMV. The analysis showed that BSV was present in nine of the ten analyzed provinces. The frequency of the virus in the 1409 collected samples was 21,4 %, the majority originated from asymptomatic plants. The species with the highest frequencies were BSOLV and BSGFV present in 9,0 % and 8,2 % of the samples, respectively while BSMYV an BSIMV were detected in 0,1 % and 0,4 % of the samples. Three FHIA hybrids showed the highest frequencies of  infected samples: FHIA 20 with 76,9 % (20/26), FHIA 25 with 46,5 % (20/43) and FHIA 21 with 42,2 % (225/533) while FHIA 18 had a 6,9 % (20/291) of infected samples and FHIA 03 only 2,8 % (3/10). Plantains Macho ¾ and CEMSA ¾ reached 10,3 % (3/29) and 4,9 % (5/102) of infected samples, respectively. Pelipita was the only cultivar among ABB hybrids to be infected with a frequency of 21,7 % (5/23). These results show that BSV has a high prevalence and a wide distribution in interspecific hybrids grown in Cuba.