Identification of Lactobacillus spp. on Basis Morphological, Physiological, and Biochemical Characteristic from Jawa Super Chicken Excreta

The use of antibiotic growth promoter as a feed additive for animals in Indonesia has been prohibited and regulated in law, starting from January 1, 2018. Alternative replacement for antibiotic was investigatedand introduced to farms such as probiotic. The commonly used probiotic was from the genus Lactobacillus, Bifidobacterium , and Bacillus. The aim of this study was to identify the Lactobacillus strains isolates from Jawa Super chicken excreta. Identification was based on the morphological, physiological, and biochemical characteristics. The sample was collected from Jawa Super chicken in Kalasan, Sleman, Yogyakarta. Each sample was diluted in 0.9% sterile saline and plated on MRS agar containing bromocresol purple. All plates were incubated at 37°C under anaerobic condition. Isolates were identified morphology, physiology, and biochemistry based on fermentation of carbohydrate by using Analytical Profile Index (API 50 CHL). This study showed that isolates belong to 2 categories of fermentation, facultatively heterofermentative and obligately heterofermentative. Not all isolates can grow at 10°C, but survive to low pH. Based on biochemical analysis isolates identify as L. brevis and L. pentosus . L. brevis and L. pentosus were strains of the Lactobacillus found in the excreta.