Purification Efficacy of Synthetic Cannabinoid Conjugates Using High-Pressure Liquid Chromatography

Abstract : In the current study, we successfully purified several synthetic cannabinoid (SC):dark quencher conjugates essential for the success of the synthetic cannabinoid detection platform developed at the US Army Research Laboratorys Weapons and Materials Research Directorate. The purification was conducted using high-pressure liquid chromatography and gradient screens to determine the most effective means of purifying the SC:dark quencher conjugates to obtain the highest yields and purity. The purity was verified using liquid chromatographycoupled mass spectroscopy and nuclear magnetic resonance.