DETERMINATION OF DRY MATTER PERCENTAGE OF EJACULATED AND EPIDIDYMAL BULL SPERMATOZOA AFTER DIFFERENTIAL FLOTATION

Previous estimates of dry matter content in spermatozoa have been variable (Ray Sarkar, Luecke & Duncan, 1947; VanDemark, 1948; Barer, Ross & Tkaczyk, 1953) but some of the methods employed might have altered the chemical composition of the spermatozoa. Therefore, a method was needed that would facilitate the separation of spermatozoa from seminal plasma and, at the same time, would ensure minimal exchange of water and other substances to and from the cells. Ballentine & Burford (1960) have separated, simply and effectively, various cells from their suspending medium by differential flotation (df). Our previous studies on the specific gravity (sp.gr.) and density distribution of bull sperma-