Rapid and Efficient Procedure for Isolation of High Yielding DNA from Castor ( Ricinus communis L.)

The ability to perform high throughput seed DNA extractions is highly desirable and essential for plant breeding and other molecular screening techniques. DNA extraction methods for PCR quality DNA from calluses and plants are not time efficient, since they require that the tissues be ground in liquid nitrogen, followed by precipitation of the DNA pellet in ethanol, washing and drying the pellet, etc. Secondly, it gets more difficult to isolate the DNA from seed containing oil. The need for a rapid and simple procedure is urgent, especially when hundreds of samples need to be analyzed. Hence, for this purpose, a simple and efficient method of isolating high quality genomic DNA for PCR amplification and enzyme digestion from seed is developed.